Supernatant was removed and beads had been washed with zero.1 M sodium phosphate, pH 6.2 . Beads had been activated by suspending in NaP with 5 mg/mL of EDC (1-ethyl-3-[3-dimethyl minutes propyl] carbodiimide hydrochloride) and 5 mg/mL sulfo-NHS (sulfo N-hydroxysulfosuccinimide) and incubated with rotation for 20 min at room temperature protected against mild. Beads were…
